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人类S100A12及其用于免疫细胞刺激的Ion诱导寡聚物的纯化
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
DOI:

12:55 min

September 29, 2019

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Chapters

  • 00:05Title
  • 01:02Anion-exchange Chromatography
  • 03:53Calcium-dependent Hydrophobic-interaction Chromatography (HIC)
  • 05:26Removal of Endotoxin and Oligomer Separation
  • 07:20Functional Testing on Monocytes
  • 10:22Results: Oligomers Separation
  • 11:59Conclusion

Summary

自动翻译

该协议描述了一种用于重组无标记标记的钙结合蛋白S100A12及其用于人类单核细胞刺激测定的由水因诱导的寡聚物的纯化方法。

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