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JoVE Journal Bioengineering
A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
 

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Article DOI: 10.3791/60317-v • 07:55 min • January 7th, 2020
January 7th, 2020 •
1, 1, 1
1Department of Chemical Engineering, Queen's University

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This protocol provides researchers with a rapid, indirect method of measuring TLR-dependent NF-кB/AP-1 transcription factor activity in a murine macrophage cell line in response to a variety of polymeric surfaces and adsorbed protein layers that model the biomaterial implant microenvironment.

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Macrophage Reporter Cell Assay Toll-like Receptor NF-kB Signaling AP-1 Signaling Adsorbed Protein Layers Polymeric Surfaces Enzymatic Assay PMMA Chloroform Glass Scintillation Vial Magnetic Stir Bar Borosilicate Glass Microscope Slide Spin Coater 70% Ethanol-sprayed Clean Box Eight-chamber Sticky Wells
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