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Immunology and Infection

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JoVE Journal Immunology and Infection
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
 

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Article DOI: 10.3791/61099-v • 10:05 min • May 22nd, 2020
May 22nd, 2020 •
*1, *1,2, 1,3
1Université Pierre et Marie Curie Paris, Sorbonne Université, Institut du Cerveau et de la Moelle Epinière, 2Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 3Department of Neurology, Yale University School of Medicine
* These authors contributed equally

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Automatic Translation

A protocol is presented combining tissue clearing with light sheet fluorescence microscopy (LSFM) to obtain three-dimensional and cellular resolution images of the lymphatic vessels and lymph nodes (LNs) collecting the cerebrospinal fluid (CSF) and spinal epidural fluid.

Tags

Three-dimensional Imaging Vertebral Lymphatic Vasculature Drainage IDISCO+ Light Sheet Fluorescence Microscopy Protocol Large-scale Views Lymphatic Vessels Immune Cells Tissue Integrity Neural Cells Vascular Cells Immune Cells Spinal Cord Meninges Epidural Space Draining Lymph Nodes Anesthetized Mouse Stereotaxic Frame Skin Incision Spinal Adapter Paracervical Muscles Paraspinal Muscles Dura Mater Arachnoid Glass Capillary Tip Cannula Fluorescent Tracer
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