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Immunology and Infection

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JoVE Journal Immunology and Infection
Modellering Hepatitis B Virus Infektion i ikke-hepatisk 293T-NE-3NRs celler
 
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Modellering Hepatitis B Virus Infektion i ikke-hepatisk 293T-NE-3NRs celler

Article DOI: 10.3791/61414-v • 09:02 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3,4, 1,2,3, 1,2,3,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications
* These authors contributed equally

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Dette manuskript beskriver en detaljeret protokol for Hepatitis B-virus (HBV) infektion i nye manipuleret 293T celler (293T-NE-3NRs, der udtrykker menneskelige NTCP, HNF4α, RXRα og PPARα) og traditionelle leverceller (HepG2-NE, der udtrykker menneskelige NTCP).

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Denne måned i JoVE HBV infektion model HepG2.2.15 HepG-NE 293T-NE-3NRs NTCP nuklearhormon receptorer
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