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纳米材料获得的蛋白质和代谢物科罗纳的毛细电电量谱方法
JoVE 杂志
化学
This content is Free Access.
JoVE 杂志 化学
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
DOI:

07:54 min

October 27, 2020

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Chapters

  • 00:04Introduction
  • 00:30Biomolecular Corona Formation and Protein Corona Isolation
  • 02:52Metabolite Corona Isolation
  • 03:50Preparation of CESI Capillaries and CE-MS for Protein Corona Analysis
  • 05:11Preparation of CESI Capillaries and CESI-MS for Metabolite Corona Analysis
  • 06:27Results: Mass Spectral Analysis of the Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
  • 07:26Conclusion

Summary

自动翻译

在这里,我们提出了一个协议,以描述完整的生物分子日冕,蛋白质和代谢物,从生物流体的纳米材料使用毛细血管电泳 - 质谱法获得。

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