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An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation
 

An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation

Article DOI: 10.3791/61812-v • 05:42 min • October 25th, 2021
October 25th, 2021 •
1, 3, 1,2
1Department of Pediatrics, Johns Hopkins University School of Medicine, 2Department of Physiology, Temple University School of Medicine, 3Department of Pediatrics, Seattle Children’s Hospital

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Automatic Translation

Primary hepatocytes are a valuable tool to study liver response and metabolism in vitro. Utilizing commercially available reagents, an improved time- and labor-efficient protocol for mouse primary hepatocyte isolation was developed.

Tags

Mouse Primary Hepatocyte Isolation Glucose Metabolism Hepatic Drug Testing Time-efficient Protocol Labor-efficient Protocol Peristaltic Pump Double Distilled Water Warmed Up Perfusion Medium C57 Black 6J Female Mouse 24 Gauge Catheter Portal Vein Inferior Vena Cava Perfusion Medium
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