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Celfractionering van U937-cellen door isopycnic density gradient purification
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
DOI:

10:19 min

August 12, 2021

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Chapters

  • 00:04Introduction
  • 00:37Cytosolic Protein Isolation
  • 02:45Cell Homogenization
  • 03:55Debris Removal and Crude Mitochondrial and Membrane Fraction Isolation
  • 04:47Isopycnic Density Gradient Purification
  • 06:34Nuclear Protein Isolation
  • 07:57Results: Fractionation of U937 Cells by Isopycnic Density Gradient Purification
  • 09:34Conclusion

Summary

自动翻译

Dit fractioneringsprotocol stelt onderzoekers in staat om cytoplasmatische, nucleaire, mitochondriale en membraaneiwitten uit zoogdiercellen te isoleren. De laatste twee subcellulaire fracties worden verder gezuiverd via isopycnic dichtheidsgradiënt.

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