Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Determination of Tripartite Interaction between…
JoVE Journal
Biology
Author Produced

需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。

JoVE Journal Biology
Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay
 

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

Article DOI: 10.3791/62791-v • 14:34 min • December 25th, 2021
December 25th, 2021 •
*1, *1, 1, 1, 1, 1, 2, 1
1Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, 2University School of Biotechnology, Guru Gobind Singh Indraprastha University
* These authors contributed equally

章节

总结概括
Automatic Translation

Here we present, a method to visualize ternary complex formation between three protein partners using fluorescent-tagged proteins by BiFC based FRET-FLIM assay. This method is valuable for studying protein-protein interaction complexes in vivo.

Tags

Protein-protein Interactions MADS-box Transcription Factor Calcium Sensor Protein BiFC-FRET-FLIM Assay Fluorescence Lifetime Measurements Tripartite Interaction Homodimerize Cytoplasm Split YFP Proteins FRET Acceptor FRET Donor Amplification Of Coding Sequences PCR Cloning Entry Vectors Antibiotic Selection Restriction Digest DNA Sequencing Destination Vectors
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter