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Journal / Biochemistry / Absolute Quantitation of Inositol Pyrophosphates…
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Biochemistry

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JoVE Journal Biochemistry
Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
 

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Article DOI: 10.3791/62847-v • 09:22 min • August 13th, 2021
August 13th, 2021 •
1, 1, 2, 1
1Institute of Organic Chemistry, University of Freiburg, 2Medical Research Council, Laboratory for Molecular Cell Biology, University College London

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A procedure for capillary electrophoresis electrospray ionization mass spectrometry for the absolute quantitation of inositol pyrophosphates from mammalian cell extracts is described.

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Absolute Quantitation Inositol Pyrophosphates Capillary Electrophoresis Electrospray Ionization Mass Spectrometry High-quality Results Inositol Phosphate Metabolism Cellular Biosynthetic Pathway CE-ESI-MS System Triple Quadrupole Tandem Mass Spectrometer Agilent Jet Stream ESI Source MassHunter Workstation CE Running Buffer Sheath Liquid Capillary Column Cutter
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