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Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
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医学
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JoVE 杂志 医学
Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
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Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

DOI:
10.3791/62995-v

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06:32 min

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April 13, 2022

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1Department of Internal Medicine,University of Texas Southwestern Medical Center, 2Charles and Jane Pak Center for Mineral Metabolism and Clinical Research,University of Texas Southwestern Medical Center

Chapters

  • 00:04Introduction
  • 00:57Amino Acid Uptake in Cells
  • 03:02Amino Acid Uptake in Freshly Isolated Bone Tissues
  • 04:32Results: L-[3,4-3H]-Glutamine Uptake in In Vitro and L-[2,3,4-3H]-Arginine Uptake Ex Vivo
  • 05:57Conclusion

Summary

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自动翻译

This protocol presents a radiolabeled amino acid uptake assay, which is useful for evaluating amino acid consumption either in primary cells or in isolated bones.

Tags

Amino Acid UptakeBone CellsBone ShaftsRadiolabeled Amino AcidsOsteoblastCell CultureST2 CellsKrebs-Ringer’s HEPESTritiated GlutamineSodium Dodecyl SulfateScintillation CounterCell CountingBone MarrowDecellularization

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