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Naturlig transformation, proteinekspression og kryokonservering af den filamentøse cyanobakterie Phormidium lacuna
 
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Naturlig transformation, proteinekspression og kryokonservering af den filamentøse cyanobakterie Phormidium lacuna

Article DOI: 10.3791/63470-v • 11:47 min • February 1st, 2022
February 1st, 2022 •
1, 1, 1, 1, 2, 2, 3, 3, 1
1Botanical Institute, Karlsruhe Institute of Technology KIT, 2Institute for Biological Interfaces 5 (IBG 5), Karlsruhe Institute of Technology KIT, 3Institut für Technische Mikrobiologie, Hochschule Mannheim

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Phormidium lacuna er en trådformede cyanobakterie, der blev isoleret fra marine rockpools. Denne artikel beskriver isolering af filamenter fra naturlige kilder, DNA-ekstraktion, genomsekventering, naturlig transformation, ekspression af sfGFP, kryokonservering og motilitetsmetoder.

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