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Immunology and Infection

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JoVE Journal Immunology and Infection
Adskillelse af immuncelleunderpopulationer i perifere blodprøver fra børn med infektiøs mononukleose
 
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Adskillelse af immuncelleunderpopulationer i perifere blodprøver fra børn med infektiøs mononukleose

Article DOI: 10.3791/64212-v • 08:44 min • September 7th, 2022
September 7th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences

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Vi beskriver en metode, der kombinerer immunmagnetiske perler og fluorescensaktiveret cellesortering for at isolere og analysere definerede immuncelleunderpopulationer af mononukleære celler i perifert blod (monocytter, CD4 + T-celler, CD8 + T-celler, B-celler og naturlige dræberceller). Ved hjælp af denne metode kan magnetiske og fluorescerende mærkede celler renses og analyseres.

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Immunologi og infektion udgave 187 Epstein-Barr-virus (EBV) infektiøs mononukleose (IM) immunomagnetisk perlesortering fluorescerende aktiveret cellesortering (FACS) immuncelleunderpopulationer
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