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使用 体内 成像筛选哺乳动物宿主活动性感染期间 白色念珠 菌突变菌株的形态发生表型
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host
DOI:

09:24 min

October 12, 2022

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Chapters

  • 00:04Introduction
  • 00:52Candida albicans Strains and Animal Preparation
  • 02:08Hair Removal and Inoculation
  • 04:06Quantify In Vitro Morphogenesis for Comparison to In Vivo Results
  • 04:42Preparation for In Vivo Imaging
  • 06:37Imaging
  • 07:38Manual Two‐Dimensional Analysis: Frequency of Filamentation
  • 07:59Results: Analyzing In Vitro and In Vivo Morphology of the Mutant Strains
  • 08:51Conclusion

Summary

自动翻译

本手稿描述了一种使用非侵入性共聚焦显微镜在哺乳动物宿主活动性感染期间筛选中等大小的 白色念珠菌 突变文库的形态发生表型的方法。

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