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Optimizing Sample Preparation Process for Transmission Electron Microscopy of Neuromuscular Junctions in Drosophila Larvae
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Optimizing Sample Preparation Process for Transmission Electron Microscopy of Neuromuscular Junctions in Drosophila Larvae

Optimizing Sample Preparation Process for Transmission Electron Microscopy of Neuromuscular Junctions in Drosophila Larvae

DOI:
10.3791/64934-v

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05:54 min

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September 15, 2023

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, , , ,
1The School of Medicine,Southeast University, 2The Key Laboratory of Developmental Genes and Human Disease,Southeast University, 3The School of Life Science and Technology,Southeast University

Chapters

  • 00:00Introduction
  • 00:59Sample Preparation for Transmission Electron Microscopy of Drosophila Larva Body Wall and Neuromuscular Junctions
  • 04:36Results: Visualizing Drosophila Larva Neuromuscular Junctions
  • 05:19Conclusion

Summary

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This report provides a new sample preparation procedure for visualizing neuromuscular junctions in Drosophila Larvae. This method is more effective in preventing the curling of the samples compared to the traditional method and is particularly useful for Drosophila neuromuscular junction ultrastructural analysis.

Tags

DrosophilaNeuromuscular JunctionTransmission Electron MicroscopySample PreparationUltrastructural AnalysisConfocal MicroscopyMuscleEmbeddingSectioning

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