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Developmental Biology
Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells (Video) | JoVE
Published: November 10, 2023
doi:
10.3791/200860
Naomi J. M. Kakiailatu
,
Laura E. Kuil
2
,
Eric Bindels
,
Joke T. M. Zink
,
Michael Vermeulen
,
Veerle Melotte
,
Maria M. Alves
1
Department of Clinical Genetics
,
Erasmus University Medical Center – Sophia Children’s Hospital
,
2
Division of Psychosocial Research and Epidemiology
,
the Netherlands Cancer Institute
,
3
Department of Hematology
,
Erasmus Medical Center
,
4
Department of Pathology, GROW-School for Oncology and Developmental Biology
,
Maastricht University Medical Center
Tags
Keywords: Gut
Zebrafish Larvae
FACS
Cell Isolation
Dissociation
Papain
Agarose Plate
HEPES
E3 Medium
Dissection
Intestine
Cell Strainer
Centrifugation
DAPI
Cell Labeling
check_url/de/200860?article_type=t
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Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells.
J. Vis. Exp.
(Pending Publication), e200860, doi:10.3791/200860 (2023).
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