Luke Alphey Oxitec Ltd Biography Publications Institution JoVE Articles Luke Alphey has not added a biography. If you are Luke Alphey and would like to personalize this page please email our Author Liaison for assistance. Publications PNUTS/PP1 Regulates RNAPII-mediated Gene Expression and is Necessary for Developmental Growth PLoS Genetics. Oct, 2013 | Pubmed ID: 24204300 Genetic Control of Mosquitoes Annual Review of Entomology. Oct, 2013 | Pubmed ID: 24160434 DsRed2 Transient Expression in Culex Quinquefasciatus Mosquitoes Memórias Do Instituto Oswaldo Cruz. Jun, 2013 | Pubmed ID: 23828005 Genetic Control of Aedes Mosquitoes Pathogens and Global Health. Jun, 2013 | Pubmed ID: 23816508 Engineered Female-specific Lethality for Control of Pest Lepidoptera ACS Synthetic Biology. Mar, 2013 | Pubmed ID: 23802263 Fitness of Transgenic Mosquito Aedes Aegypti Males Carrying a Dominant Lethal Genetic System PloS One. 2013 | Pubmed ID: 23690948 Development of a Population Suppression Strain of the Human Malaria Vector Mosquito, Anopheles Stephensi Malaria Journal. 2013 | Pubmed ID: 23622561 Transgene-based, Female-specific Lethality System for Genetic Sexing of the Silkworm, Bombyx Mori Proceedings of the National Academy of Sciences of the United States of America. Apr, 2013 | Pubmed ID: 23569267 Oral Ingestion of Transgenic RIDL Ae. Aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species PloS One. 2013 | Pubmed ID: 23527029 The Orthologue of the Fruitfly Sex Behaviour Gene Fruitless in the Mosquito Aedes Aegypti: Evolution of Genomic Organisation and Alternative Splicing PloS One. 2013 | Pubmed ID: 23418412 Polymerase Chain Displacement Reaction BioTechniques. Feb, 2013 | Pubmed ID: 23384180 Field Cage Studies and Progressive Evaluation of Genetically-engineered Mosquitoes PLoS Neglected Tropical Diseases. 2013 | Pubmed ID: 23350003 Mating Compatibility and Competitiveness of Transgenic and Wild Type Aedes Aegypti (L.) Under Contained Semi-field Conditions Transgenic Research. Feb, 2013 | Pubmed ID: 22700207 Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton PloS One. 2012 | Pubmed ID: 23226548 Open Field Release of Genetically Engineered Sterile Male Aedes Aegypti in Malaysia PloS One. 2012 | Pubmed ID: 22970102 Successful Suppression of a Field Mosquito Population by Sustained Release of Engineered Male Mosquitoes Nature Biotechnology. Sep, 2012 | Pubmed ID: 22965050 Flight Performance and Teneral Energy Reserves of Two Genetically-modified and One Wild-type Strain of the Yellow Fever Mosquito Aedes Aegypti Vector Borne and Zoonotic Diseases (Larchmont, N.Y.). Dec, 2012 | Pubmed ID: 22835152 Female-specific Flightless (fsRIDL) Phenotype for Control of Aedes Albopictus PLoS Neglected Tropical Diseases. 2012 | Pubmed ID: 22802980 Control of the Olive Fruit Fly Using Genetics-enhanced Sterile Insect Technique BMC Biology. 2012 | Pubmed ID: 22713628 Field Longevity of a Fluorescent Protein Marker in an Engineered Strain of the Pink Bollworm, Pectinophora Gossypiella (Saunders) PloS One. 2012 | Pubmed ID: 22693645 DengueTools: Innovative Tools and Strategies for the Surveillance and Control of Dengue Global Health Action. 2012 | Pubmed ID: 22451836 Appropriate Regulation of GM Insects PLoS Neglected Tropical Diseases. Jan, 2012 | Pubmed ID: 22303495 Multiplex Detection and SNP Genotyping in a Single Fluorescence Channel PloS One. 2012 | Pubmed ID: 22272339 Field Performance of Engineered Male Mosquitoes Nature Biotechnology. Nov, 2011 | Pubmed ID: 22037376 Cost of Mating and Insemination Capacity of a Genetically Modified Mosquito Aedes Aegypti OX513A Compared to Its Wild Type Counterpart PloS One. 2011 | Pubmed ID: 22022518 A Model Framework to Estimate Impact and Cost of Genetics-based Sterile Insect Methods for Dengue Vector Control PloS One. 2011 | Pubmed ID: 21998654 Field Performance of a Genetically Engineered Strain of Pink Bollworm PloS One. 2011 | Pubmed ID: 21931649 Comparison of Life History Characteristics of the Genetically Modified OX513A Line and a Wild Type Strain of Aedes Aegypti PloS One. 2011 | Pubmed ID: 21698096 Why RIDL is Not SIT Trends in Parasitology. Aug, 2011 | Pubmed ID: 21659002 Genetic Elimination of Dengue Vector Mosquitoes Proceedings of the National Academy of Sciences of the United States of America. Mar, 2011 | Pubmed ID: 21383140 Modeling Resistance to Genetic Control of Insects Journal of Theoretical Biology. Feb, 2011 | Pubmed ID: 21075122 Irritant and Repellent Behavioral Responses of Aedes Aegypti Male Populations Developed for RIDL Disease Control Strategies Journal of Medical Entomology. Nov, 2010 | Pubmed ID: 21175058 Use of a Regulatory Mechanism of Sex Determination in Pest Insect Control Journal of Genetics. Sep, 2010 | Pubmed ID: 20876996 Piggybac- and PhiC31-mediated Genetic Transformation of the Asian Tiger Mosquito, Aedes Albopictus (Skuse) PLoS Neglected Tropical Diseases. 2010 | Pubmed ID: 20808959 Transcriptomics and Disease Vector Control BMC Biology. 2010 | Pubmed ID: 20525113 Female-specific Flightless Phenotype for Mosquito Control Proceedings of the National Academy of Sciences of the United States of America. Mar, 2010 | Pubmed ID: 20176967 Sterile-insect Methods for Control of Mosquito-borne Diseases: an Analysis Vector Borne and Zoonotic Diseases (Larchmont, N.Y.). Apr, 2010 | Pubmed ID: 19725763 Sex Separation Strategies: Past Experience and New Approaches Malaria Journal. 2009 | Pubmed ID: 19917075 Combining Pest Control and Resistance Management: Synergy of Engineered Insects with Bt Crops Journal of Economic Entomology. Apr, 2009 | Pubmed ID: 19449654 Natural and Engineered Mosquito Immunity Journal of Biology. 2009 | Pubmed ID: 19439051 Interview with Luke Alphey, Ph.D. [Interview by Vicki Glaser] Vector Borne and Zoonotic Diseases (Larchmont, N.Y.). Apr, 2009 | Pubmed ID: 19371156 Insect Population Suppression Using Engineered Insects Advances in Experimental Medicine and Biology. 2008 | Pubmed ID: 18510017 Drosophila Uri, a PP1alpha Binding Protein, is Essential for Viability, Maintenance of DNA Integrity and Normal Transcriptional Activity BMC Molecular Biology. 2008 | Pubmed ID: 18412953 Managing Insecticide Resistance by Mass Release of Engineered Insects Journal of Economic Entomology. Oct, 2007 | Pubmed ID: 17972643 Genetic Sexing Through the Use of Y-linked Transgenes Insect Biochemistry and Molecular Biology. Nov, 2007 | Pubmed ID: 17916503 Analyzing the Control of Mosquito-borne Diseases by a Dominant Lethal Genetic System Proceedings of the National Academy of Sciences of the United States of America. May, 2007 | Pubmed ID: 17519336 Essential, Overlapping and Redundant Roles of the Drosophila Protein Phosphatase 1 Alpha and 1 Beta Genes Genetics. May, 2007 | Pubmed ID: 17513890 Late-acting Dominant Lethal Genetic Systems and Mosquito Control BMC Biology. 2007 | Pubmed ID: 17374148 Female-specific Insect Lethality Engineered Using Alternative Splicing Nature Biotechnology. Mar, 2007 | Pubmed ID: 17322873 The Nonmuscle Myosin Phosphatase PP1beta (flapwing) Negatively Regulates Jun N-terminal Kinase in Wing Imaginal Discs of Drosophila Genetics. Apr, 2007 | Pubmed ID: 17277363 Yeast Two-hybrid Screens to Identify Drosophila PP1-binding Proteins Methods in Molecular Biology (Clifton, N.J.). 2007 | Pubmed ID: 17200561 Towards a Comprehensive Analysis of the Protein Phosphatase 1 Interactome in Drosophila Journal of Molecular Biology. Nov, 2006 | Pubmed ID: 17007873 Transposon-free Insertions for Insect Genetic Engineering Nature Biotechnology. Jul, 2006 | Pubmed ID: 16823373 CG15031/PPYR1 is an Intrinsically Unstructured Protein That Interacts with Protein Phosphatase Y Archives of Biochemistry and Biophysics. Jul, 2006 | Pubmed ID: 16631104 Mosquito Transgenesis: What is the Fitness Cost? Trends in Parasitology. May, 2006 | Pubmed ID: 16564223 Germ Line Specific Expression of a Protein Phosphatase Y Interacting Protein (PPYR1) in Drosophila Gene Expression Patterns : GEP. Oct, 2006 | Pubmed ID: 16530491 Bifocal and PP1 Interaction Regulates Targeting of the R-cell Growth Cone in Drosophila Developmental Biology. Dec, 2005 | Pubmed ID: 16280124 A Dominant Lethal Genetic System for Autocidal Control of the Mediterranean Fruitfly Nature Biotechnology. Apr, 2005 | Pubmed ID: 15750586 PP1beta9C Interacts with Trithorax in Drosophila Wing Development Developmental Dynamics : an Official Publication of the American Association of Anatomists. Oct, 2004 | Pubmed ID: 15366010 The Essential Role of PP1beta in Drosophila is to Regulate Nonmuscle Myosin Molecular Biology of the Cell. Oct, 2004 | Pubmed ID: 15269282 Cloning and Expression of Mars, a Novel Member of the Guanylate Kinase Associated Protein Family in Drosophila Gene Expression Patterns : GEP. Sep, 2004 | Pubmed ID: 15261830 Genetic Control of Vector Populations: an Imminent Prospect Tropical Medicine & International Health : TM & IH. Apr, 2004 | Pubmed ID: 15078260 Giant Nuclei is Essential in the Cell Cycle Transition from Meiosis to Mitosis Development (Cambridge, England). Jul, 2003 | Pubmed ID: 12756181 Ectopic Expression of Inhibitors of Protein Phosphatase Type 1 (PP1) Can Be Used to Analyze Roles of PP1 in Drosophila Development Genetics. May, 2003 | Pubmed ID: 12750335 Trithorax Interacts with Type 1 Serine/threonine Protein Phosphatase in Drosophila EMBO Reports. Jan, 2003 | Pubmed ID: 12524522 Malaria Control with Genetically Manipulated Insect Vectors Science (New York, N.Y.). Oct, 2002 | Pubmed ID: 12364786 Functional Interaction Between Nuclear Inhibitor of Protein Phosphatase Type 1 (NIPP1) and Protein Phosphatase Type 1 (PP1) in Drosophila: Consequences of Over-expression of NIPP1 in Flies and Suppression by Co-expression of PP1 The Biochemical Journal. Dec, 2002 | Pubmed ID: 12358598 Re-engineering the Sterile Insect Technique Insect Biochemistry and Molecular Biology. Oct, 2002 | Pubmed ID: 12225915 PP1 Binds Sara and Negatively Regulates Dpp Signaling in Drosophila Melanogaster Nature Genetics. Aug, 2002 | Pubmed ID: 12134149 Dominant Lethality and Insect Population Control Molecular and Biochemical Parasitology. May, 2002 | Pubmed ID: 12034450 Mass Production of Genetically Modified Aedes aegypti for Field Releases in Brazil Danilo O. Carvalho1,2, Derric Nimmo1, Neil Naish1, Andrew R. McKemey1, Pam Gray1, André B. B. Wilke3, Mauro T. Marrelli3, Jair F. Virginio4, Luke Alphey1,5, Margareth L. Capurro2,6 1Oxitec Ltd, 2Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 3Departamento de Epidemiologia, Universidade de São Paulo, 4Moscamed Brasil, 5Deptartment of Zoology, University of Oxford, 6Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM) JoVE 3579 Umwelt
Mass Production of Genetically Modified Aedes aegypti for Field Releases in Brazil Danilo O. Carvalho1,2, Derric Nimmo1, Neil Naish1, Andrew R. McKemey1, Pam Gray1, André B. B. Wilke3, Mauro T. Marrelli3, Jair F. Virginio4, Luke Alphey1,5, Margareth L. Capurro2,6 1Oxitec Ltd, 2Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 3Departamento de Epidemiologia, Universidade de São Paulo, 4Moscamed Brasil, 5Deptartment of Zoology, University of Oxford, 6Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM) JoVE 3579 Umwelt