Robert P. Mecham Department of Cell Biology & Physiology Washington University School of Medicine Biography Publications Institution JoVE Articles Robert P. Mecham has not added a biography. If you are Robert P. Mecham and would like to personalize this page please email our Author Liaison for assistance. Publications Loss of Angiotensin II Type 2 Receptor Improves Blood Pressure in Elastin Insufficiency Frontiers in Cardiovascular Medicine. 2021 | Pubmed ID: 34790711 Deletion of Type VIII Collagen Reduces Blood Pressure, Increases Carotid Artery Functional Distensibility and Promotes Elastin Deposition Matrix Biology Plus. Dec, 2021 | Pubmed ID: 34693248 Vascular Smooth Muscle Cell Subpopulations and Neointimal Formation in Mouse Models of Elastin Insufficiency Arteriosclerosis, Thrombosis, and Vascular Biology. 12, 2021 | Pubmed ID: 34587758 Tissue-specific Smooth Muscle Cell Subtypes Identified by Transcriptional Profiling The International Journal of Biochemistry & Cell Biology. 10, 2021 | Pubmed ID: 34411694 JNK2 Gene Silencing for Elastic Matrix Regenerative Repair Tissue Engineering. Part A. Aug, 2021 | Pubmed ID: 34409851 Inhibition of NOX1 Mitigates Blood Pressure Increases in Elastin Insufficiency Function (Oxford, England). 2021 | Pubmed ID: 34223172 SVEP1 is a Human Coronary Artery Disease Locus That Promotes Atherosclerosis Science Translational Medicine. 03, 2021 | Pubmed ID: 33762433 Complex Consequences of Cantu Syndrome SUR2 Variant R1154Q in Genetically Modified Mice JCI Insight. 03, 2021 | Pubmed ID: 33529173 RNA Vaccines for COVID-19: 5 Things Every Cardiologist Should Know JACC. Basic to Translational Science. Dec, 2020 | Pubmed ID: 33251385 A Matricellular Protein Fibulin-4 is Essential for the Activation of Lysyl Oxidase Science Advances. Nov, 2020 | Pubmed ID: 33239290 Whole Exome Sequencing in Patients with Williams-Beuren Syndrome Followed by Disease Modeling in Mice Points to Four Novel Pathways That May Modify Stenosis Risk Human Molecular Genetics. May, 2020 | Pubmed ID: 32412588 Identification of the Growth Factor-binding Sequence in the Extracellular Matrix Protein MAGP-1 The Journal of Biological Chemistry. 02, 2020 | Pubmed ID: 31988245 Elastic Fiber Ultrastructure and Assembly Matrix Biology : Journal of the International Society for Matrix Biology. 11, 2019 | Pubmed ID: 31669522 Sodium-activated Potassium Channels Moderate Excitability in Vascular Smooth Muscle The Journal of Physiology. 10, 2019 | Pubmed ID: 31444905 Intracellular Retention of Mutant Lysyl Oxidase Leads to Aortic Dilation in Response to Increased Hemodynamic Stress JCI Insight. 06, 2019 | Pubmed ID: 31211696 Decreased Lysyl Oxidase Level Protects Against Development of Retinal Vascular Lesions in Diabetic Retinopathy Experimental Eye Research. 07, 2019 | Pubmed ID: 31022398 Cardiovascular Consequences of KATP Overactivity in Cantu Syndrome JCI Insight. 08, 2018 | Pubmed ID: 30089727 Extracellular Determinants of Arterial Morphogenesis, Growth, and Homeostasis Current Topics in Developmental Biology. 2018 | Pubmed ID: 29853177 Spatial and Temporal Changes in Extracellular Elastin and Laminin Distribution During Lung Alveolar Development Scientific Reports. 05, 2018 | Pubmed ID: 29844468 Requisite Endothelial Reactivation and Effective SiRNA Nanoparticle Targeting of Etv2/Er71 in Tumor Angiogenesis JCI Insight. 04, 2018 | Pubmed ID: 29669933 Microfibril-associated Glycoproteins MAGP-1 and MAGP-2 in Disease Matrix Biology : Journal of the International Society for Matrix Biology. 10, 2018 | Pubmed ID: 29524629 Contribution of Metabolic Disease to Bone Fragility in MAGP1-deficient Mice Matrix Biology : Journal of the International Society for Matrix Biology. 04, 2018 | Pubmed ID: 29519758 Elastin in Lung Development and Disease Pathogenesis Matrix Biology : Journal of the International Society for Matrix Biology. 11, 2018 | Pubmed ID: 29331337 Elastin Purification and Solubilization Methods in Cell Biology. 2018 | Pubmed ID: 29310779 Measurement of Elastin, Collagen, and Total Protein Levels in Tissues Methods in Cell Biology. 2018 | Pubmed ID: 29310774 FGF Receptors Control Alveolar Elastogenesis Development (Cambridge, England). 12, 2017 | Pubmed ID: 29122839 Downregulation of Lysyl Oxidase Protects Retinal Endothelial Cells From High Glucose-Induced Apoptosis Investigative Ophthalmology & Visual Science. 05, 2017 | Pubmed ID: 28538980 Fibulin-4 is Essential for Maintaining Arterial Wall Integrity in Conduit but Not Muscular Arteries Science Advances. May, 2017 | Pubmed ID: 28508064 Inhibition of MAPK-Erk Pathway In vivo Attenuates Aortic Valve Disease Processes in Emilin1-deficient Mouse Model Physiological Reports. Mar, 2017 | Pubmed ID: 28270590 Deficient Circumferential Growth Is the Primary Determinant of Aortic Obstruction Attributable to Partial Elastin Deficiency Arteriosclerosis, Thrombosis, and Vascular Biology. 05, 2017 | Pubmed ID: 28254817 Marrow Adipose Tissue Expansion Coincides with Insulin Resistance in MAGP1-Deficient Mice Frontiers in Endocrinology. 2016 | Pubmed ID: 27445989 Loss of Function Mutation in LOX Causes Thoracic Aortic Aneurysm and Dissection in Humans Proceedings of the National Academy of Sciences of the United States of America. 08, 2016 | Pubmed ID: 27432961 Albumin Contributes to Kidney Disease Progression in Alport Syndrome American Journal of Physiology. Renal Physiology. 07, 2016 | Pubmed ID: 27147675 Characterization of Metabolic Health in Mouse Models of Fibrillin-1 Perturbation Matrix Biology : Journal of the International Society for Matrix Biology. 09, 2016 | Pubmed ID: 26902431 Chronic Antihypertensive Treatment Improves Pulse Pressure but Not Large Artery Mechanics in a Mouse Model of Congenital Vascular Stiffness American Journal of Physiology. Heart and Circulatory Physiology. Sep, 2015 | Pubmed ID: 26232234 Fibulin-4 E57K Knock-in Mice Recapitulate Cutaneous, Vascular and Skeletal Defects of Recessive Cutis Laxa 1B with Both Elastic Fiber and Collagen Fibril Abnormalities The Journal of Biological Chemistry. Aug, 2015 | Pubmed ID: 26178373 The Microfibril-associated Glycoproteins (MAGPs) and the Microfibrillar Niche Matrix Biology : Journal of the International Society for Matrix Biology. Sep, 2015 | Pubmed ID: 25963142 Adamtsl2 Deletion Results in Bronchial Fibrillin Microfibril Accumulation and Bronchial Epithelial Dysplasia--a Novel Mouse Model Providing Insights into Geleophysic Dysplasia Disease Models & Mechanisms. May, 2015 | Pubmed ID: 25762570 Fibroblast Growth Factor 2 is Required for Epithelial Recovery, but Not for Pulmonary Fibrosis, in Response to Bleomycin American Journal of Respiratory Cell and Molecular Biology. Jan, 2015 | Pubmed ID: 24988442 The Extracellular Matrix Protein MAGP1 Supports Thermogenesis and Protects Against Obesity and Diabetes Through Regulation of TGF-β Diabetes. Jun, 2014 | Pubmed ID: 24458361 Altered Reactivity of Resistance Vasculature Contributes to Hypertension in Elastin Insufficiency American Journal of Physiology. Heart and Circulatory Physiology. Mar, 2014 | Pubmed ID: 24414067 Nonselective Assembly of Fibrillin 1 and Fibrillin 2 in the Rodent Ocular Zonule and in Cultured Cells: Implications for Marfan Syndrome Investigative Ophthalmology & Visual Science. Dec, 2013 | Pubmed ID: 24265020 Ocular Phenotype of Fbn2-null Mice Investigative Ophthalmology & Visual Science. Nov, 2013 | Pubmed ID: 24130178 Hypotension Due to Kir6.1 Gain-of-function in Vascular Smooth Muscle Journal of the American Heart Association. Aug, 2013 | Pubmed ID: 23974906 Microfibril-associated Glycoprotein 2 (MAGP2) Loss of Function Has Pleiotropic Effects in Vivo The Journal of Biological Chemistry. Oct, 2013 | Pubmed ID: 23963447 Zap70 Inhibits Syk-mediated Osteoclast Function Journal of Cellular Biochemistry. Aug, 2013 | Pubmed ID: 23494777 Development, Composition, and Structural Arrangements of the Ciliary Zonule of the Mouse Investigative Ophthalmology & Visual Science. Apr, 2013 | Pubmed ID: 23493297 A Fiber-based Constitutive Model Predicts Changes in Amount and Organization of Matrix Proteins with Development and Disease in the Mouse Aorta Biomechanics and Modeling in Mechanobiology. Jun, 2013 | Pubmed ID: 22790326 Overview of Extracellular Matrix Current Protocols in Cell Biology. Dec, 2012 | Pubmed ID: 23208544 Paxillin Contracts the Osteoclast Cytoskeleton Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research. Dec, 2012 | Pubmed ID: 22807029 Alternative Splicing and Tissue-specific Elastin Misassembly Act As Biological Modifiers of Human Elastin Gene Frameshift Mutations Associated with Dominant Cutis Laxa The Journal of Biological Chemistry. Jun, 2012 | Pubmed ID: 22573328 Oophorectomy-induced Bone Loss is Attenuated in MAGP1-deficient Mice Journal of Cellular Biochemistry. Jan, 2012 | Pubmed ID: 21898536 Inhibition of MicroRNA-29 Enhances Elastin Levels in Cells Haploinsufficient for Elastin and in Bioengineered Vessels Arteriosclerosis, Thrombosis, and Vascular Biology. Nov, 2011 | Pubmed ID: 22095981 Genetic Modifiers of Cardiovascular Phenotype Caused by Elastin Haploinsufficiency Act by Extrinsic Noncomplementation The Journal of Biological Chemistry. Dec, 2011 | Pubmed ID: 22049077 Pathogenesis of Aortic Dilatation in Mucopolysaccharidosis VII Mice May Involve Complement Activation Molecular Genetics and Metabolism. Dec, 2011 | Pubmed ID: 21944884 Characterization of Erectile Function in Elastin Haploinsufficicent Mice The Journal of Sexual Medicine. Nov, 2011 | Pubmed ID: 21883953 Decreased Aortic Diameter and Compliance Precedes Blood Pressure Increases in Postnatal Development of Elastin-insufficient Mice American Journal of Physiology. Heart and Circulatory Physiology. Jul, 2011 | Pubmed ID: 21536846 Oxidative Modifications of the C-terminal Domain of Tropoelastin Prevent Cell Binding The Journal of Biological Chemistry. Apr, 2011 | Pubmed ID: 21321118 New Insights into the Pathogenesis of Autosomal-dominant Cutis Laxa with Report of Five ELN Mutations Human Mutation. Apr, 2011 | Pubmed ID: 21309044 Evidence for Heterozygous Abnormalities of the Elastin Gene (ELN) Affecting the Quantity of Vocal Fold Elastic Fibers: a Pilot Study Journal of Voice : Official Journal of the Voice Foundation. Mar, 2011 | Pubmed ID: 20926252 Oxidative and Nitrosative Modifications of Tropoelastin Prevent Elastic Fiber Assembly in Vitro The Journal of Biological Chemistry. Nov, 2010 | Pubmed ID: 20847053 The Matrix-binding Domain of Microfibril-associated Glycoprotein-1 Targets Active Connective Tissue Growth Factor to a Fibroblast-produced Extracellular Matrix Macromolecular Bioscience. Nov, 2010 | Pubmed ID: 20799254 Mechanisms of Emphysema in Autosomal Dominant Cutis Laxa Matrix Biology : Journal of the International Society for Matrix Biology. Sep, 2010 | Pubmed ID: 20600892 Microfibril-associated Glycoprotein-1, an Extracellular Matrix Regulator of Bone Remodeling The Journal of Biological Chemistry. Jul, 2010 | Pubmed ID: 20501659 Discrete Contributions of Elastic Fiber Components to Arterial Development and Mechanical Compliance Arteriosclerosis, Thrombosis, and Vascular Biology. Dec, 2009 | Pubmed ID: 19850904 Vascular Extracellular Matrix and Arterial Mechanics Physiological Reviews. Jul, 2009 | Pubmed ID: 19584318 Effects of Chronic Treatment with a Low Dose of Nicorandil on the Function of the Rat Aorta During Ageing Clinical and Experimental Pharmacology & Physiology. Oct, 2009 | Pubmed ID: 19473347 Reduced Vessel Elasticity Alters Cardiovascular Structure and Function in Newborn Mice Circulation Research. May, 2009 | Pubmed ID: 19372465 Fibrillins, Fibulins, and Matrix-associated Glycoprotein Modulate the Kinetics and Morphology of in Vitro Self-assembly of a Recombinant Elastin-like Polypeptide Biochemistry. Nov, 2008 | Pubmed ID: 18973305 Essential Role for Fibrillin-2 in Zebrafish Notochord and Vascular Morphogenesis Developmental Dynamics : an Official Publication of the American Association of Anatomists. Oct, 2008 | Pubmed ID: 18816837 Elastin Insufficiency Predisposes to Elevated Pulmonary Circulatory Pressures Through Changes in Elastic Artery Structure Journal of Applied Physiology (Bethesda, Md. : 1985). Nov, 2008 | Pubmed ID: 18772328 Deficiency in Microfibril-associated Glycoprotein-1 Leads to Complex Phenotypes in Multiple Organ Systems The Journal of Biological Chemistry. Sep, 2008 | Pubmed ID: 18625713 Modification and Functional Inactivation of the Tropoelastin Carboxy-terminal Domain in Cross-linked Elastin Matrix Biology : Journal of the International Society for Matrix Biology. Sep, 2008 | Pubmed ID: 18602002 Upregulation of Elastase Proteins Results in Aortic Dilatation in Mucopolysaccharidosis I Mice Molecular Genetics and Metabolism. Jul, 2008 | Pubmed ID: 18479957 Methods in Elastic Tissue Biology: Elastin Isolation and Purification Methods (San Diego, Calif.). May, 2008 | Pubmed ID: 18442703 Mice Lacking the Extracellular Matrix Protein MAGP1 Display Delayed Thrombotic Occlusion Following Vessel Injury Blood. Apr, 2008 | Pubmed ID: 18281502 Neutrophil Elastase Cleaves Laminin-332 (laminin-5) Generating Peptides That Are Chemotactic for Neutrophils The Journal of Biological Chemistry. Apr, 2008 | Pubmed ID: 18178964 Elastin Haploinsufficiency Induces Alternative Aging Processes in the Aorta Rejuvenation Research. Feb, 2008 | Pubmed ID: 18173368 Fibulin-2 is Dispensable for Mouse Development and Elastic Fiber Formation Molecular and Cellular Biology. Feb, 2008 | Pubmed ID: 18070922 New Insights into Elastic Fiber Assembly Birth Defects Research. Part C, Embryo Today : Reviews. Dec, 2007 | Pubmed ID: 18228265 The Intracellular Form of Human MAGP1 Elicits a Complex and Specific Transcriptional Response The International Journal of Biochemistry & Cell Biology. 2007 | Pubmed ID: 17692555 Functional Rescue of Elastin Insufficiency in Mice by the Human Elastin Gene: Implications for Mouse Models of Human Disease Circulation Research. Aug, 2007 | Pubmed ID: 17626896 Elastin-insufficient Mice Show Normal Cardiovascular Remodeling in 2K1C Hypertension Despite Higher Baseline Pressure and Unique Cardiovascular Architecture American Journal of Physiology. Heart and Circulatory Physiology. Jul, 2007 | Pubmed ID: 17400710 Elastin Protein Levels Are a Vital Modifier Affecting Normal Lung Development and Susceptibility to Emphysema American Journal of Physiology. Lung Cellular and Molecular Physiology. Mar, 2007 | Pubmed ID: 17142349 Domains 16 and 17 of Tropoelastin in Elastic Fibre Formation The Biochemical Journal. Feb, 2007 | Pubmed ID: 17037986 Fibulin-5 Mutations: Mechanisms of Impaired Elastic Fiber Formation in Recessive Cutis Laxa Human Molecular Genetics. Dec, 2006 | Pubmed ID: 17035250 The Stumbling Block in Lung Repair of Emphysema: Elastic Fiber Assembly Proceedings of the American Thoracic Society. Jul, 2006 | Pubmed ID: 16799087 Specific Sequence Motifs Direct the Oxygenation and Chlorination of Tryptophan by Myeloperoxidase Biochemistry. Mar, 2006 | Pubmed ID: 16548523 Elastin Fragments Drive Disease Progression in a Murine Model of Emphysema The Journal of Clinical Investigation. Mar, 2006 | Pubmed ID: 16470245 Elastic Fiber Formation: a Dynamic View of Extracellular Matrix Assembly Using Timer Reporters Journal of Cellular Physiology. Apr, 2006 | Pubmed ID: 16261592 Tropoelastin Interacts with Cell-surface Glycosaminoglycans Via Its COOH-terminal Domain The Journal of Biological Chemistry. Dec, 2005 | Pubmed ID: 16192266 A Functional Mutation in the Terminal Exon of Elastin in Severe, Early-onset Chronic Obstructive Pulmonary Disease American Journal of Respiratory Cell and Molecular Biology. Oct, 2005 | Pubmed ID: 16081882 Effects of Elastin Haploinsufficiency on the Mechanical Behavior of Mouse Arteries American Journal of Physiology. Heart and Circulatory Physiology. Sep, 2005 | Pubmed ID: 15863465 Altered Vascular Remodeling in Fibulin-5-deficient Mice Reveals a Role of Fibulin-5 in Smooth Muscle Cell Proliferation and Migration Proceedings of the National Academy of Sciences of the United States of America. Feb, 2005 | Pubmed ID: 15710889 TGF-beta-dependent Pathogenesis of Mitral Valve Prolapse in a Mouse Model of Marfan Syndrome The Journal of Clinical Investigation. Dec, 2004 | Pubmed ID: 15546004 Vascular Extracellular Matrix and Aortic Development Current Topics in Developmental Biology. 2004 | Pubmed ID: 15522742 Deposition of Tropoelastin into the Extracellular Matrix Requires a Competent Elastic Fiber Scaffold but Not Live Cells Matrix Biology : Journal of the International Society for Matrix Biology. Apr, 2004 | Pubmed ID: 15172035 Neutrophil Serine Proteinases Inactivate Surfactant Protein D by Cleaving Within a Conserved Subregion of the Carbohydrate Recognition Domain The Journal of Biological Chemistry. Jun, 2004 | Pubmed ID: 15078883 Identification of a Major Microfibril-associated Glycoprotein-1-binding Domain in Fibrillin-2 The Journal of Biological Chemistry. May, 2004 | Pubmed ID: 15044481 Developmental Adaptation of the Mouse Cardiovascular System to Elastin Haploinsufficiency The Journal of Clinical Investigation. Nov, 2003 | Pubmed ID: 14597767 Dexamethasone Induction of Hypertension and Diabetes is PPAR-alpha Dependent in LDL Receptor-null Mice Nature Medicine. Aug, 2003 | Pubmed ID: 12847522 Fibrillin-1 and -2 Contain Heparin-binding Sites Important for Matrix Deposition and That Support Cell Attachment The Biochemical Journal. Oct, 2003 | Pubmed ID: 12837131 A Site on Laminin Alpha 5, AQARSAASKVKVSMKF, Induces Inflammatory Cell Production of Matrix Metalloproteinase-9 and Chemotaxis Journal of Immunology (Baltimore, Md. : 1950). Jul, 2003 | Pubmed ID: 12817023 Domains in Tropoelastin That Mediate Elastin Deposition in Vitro and in Vivo The Journal of Biological Chemistry. May, 2003 | Pubmed ID: 12626514 Hypertension and Prolonged Vasoconstrictor Signaling in RGS2-deficient Mice The Journal of Clinical Investigation. Feb, 2003 | Pubmed ID: 12588882 Lysyl Oxidase is Required for Vascular and Diaphragmatic Development in Mice The Journal of Biological Chemistry. Apr, 2003 | Pubmed ID: 12473682 The Microfibrillar Proteins MAGP-1 and Fibrillin-1 Form a Ternary Complex with the Chondroitin Sulfate Proteoglycan Decorin Molecular Biology of the Cell. May, 2000 | Pubmed ID: 10793130 Characterization of an in Vitro Model of Elastic Fiber Assembly Molecular Biology of the Cell. Nov, 1999 | Pubmed ID: 10564258 Identification of Tropoelastin As a Ligand for the 65-kD FK506-binding Protein, FKBP65, in the Secretory Pathway The Journal of Cell Biology. Jan, 1998 | Pubmed ID: 9442105 Chlamydomonas Agglutinin is a Hydroxyproline-rich Glycoprotein Proceedings of the National Academy of Sciences of the United States of America. Oct, 1983 | Pubmed ID: 16593377 التحضير البيولوجي والتقنية الميكانيكية لتحديد الخصائص اللزجة المرنة للألياف المناطقية Juan Rodriguez1,2, Matthew Reilly3, Robert P. Mecham4, Steven Bassnett2,4 1Department of Basic Sciences, University of Health Sciences and Pharmacy, 2Department of Ophthalmology & Visual Sciences, Washington University School of Medicine, 3Department of Biomedical Engineering, The Ohio State University, 4Department of Cell Biology & Physiology, Washington University School of Medicine JoVE 63171 Medizin
التحضير البيولوجي والتقنية الميكانيكية لتحديد الخصائص اللزجة المرنة للألياف المناطقية Juan Rodriguez1,2, Matthew Reilly3, Robert P. Mecham4, Steven Bassnett2,4 1Department of Basic Sciences, University of Health Sciences and Pharmacy, 2Department of Ophthalmology & Visual Sciences, Washington University School of Medicine, 3Department of Biomedical Engineering, The Ohio State University, 4Department of Cell Biology & Physiology, Washington University School of Medicine JoVE 63171 Medizin