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Biologie
분리 및 조혈 줄기 세포의 이식 (HSCs)
Published: February 25, 2007
doi:
10.3791/157
Cristina Lo Celso
,
David Scadden
1
Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Stem Cell Institute
,
Harvard Medical School
Protocol
총 골수 준비 네 B6 생쥐는 희생과 tibias, 대퇴골, 허리와 척추를 얻을 해부하고 있습니다. 해부 동안 팔다리와 뼈는 PBS 2퍼센트 FCS 열 inactivated에 보관 (옵션 2mM EDA – 해부 매체를.) 깨끗한 뼈가 해부 매체에 박격포와 유봉과 함께 깔린 있습니다. 그것을 할 효율적인 방법은 한 번에 다음 각 마우스의 tibias, 대퇴골과 엉덩이, 2 쪽이를 부술 것입니다. 각 마우스에서 얻은 ?…
Tags
Isolation
Transplantation
Hematopoietic Stem Cells
HSCs
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Diesen Artikel zitieren
Lo Celso, C., Scadden, D. Isolation and Transplantation of Hematopoietic Stem Cells (HSCs).
J. Vis. Exp.
(2), e157, doi:10.3791/157 (2007).
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