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Neurowissenschaften
Sample Mounting for Light-Sheet Fluorescence Microscopy and Stripping for Multi-Round Labeling of the Same Specimens
Published: January 26, 2024
doi:
10.3791/200807
Danila Di Meo*
1
,
Josephine Ramazzotti*
1
,
Marina Scardigli*
1,2
,
Franco Cheli
,
Luca Pesce
3
,
Niamh Brady
,
Giacomo Mazzamuto
4,5
,
Irene Costantini
4,6
,
Francesco S. Pavone
4,5
1
European Laboratory for Non-linear Spectroscopy (LENS)
,
University of Florence
,
2
Division of Physiology, Department of Experimental and Clinical Medicine
,
University of Florence
,
3
Department of Physics
,
University of Pisa
,
4
National Research Council – National Institute of Optics (CNR-INO)
,
5
Department of Physics and Astronomy
,
University of Florence
,
6
Department of Biology
,
University of Florence
Tags
Sample Mounting
Light-sheet Fluorescence Microscopy
Multi-round Labeling
Steel Spacer
Quartz Cover Slip
Silicone Glue
TDE
PBS
Clearing Solution
Broca’s Area
NeuN
Somatostatin
Claritin
Interneurons
Light Sheet Fluorescence Microscopy
Neuronal Markers
Maximum Intensity Projection
check_url/de/200807?article_type=t
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Sample Mounting for Light-Sheet Fluorescence Microscopy and Stripping for Multi-Round Labeling of the Same Specimens.
J. Vis. Exp.
(Pending Publication), e200807, doi:10.3791/200807 (2024).
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