Any DC supply would work. The specific voltage of a different machine will need further optimization.
Disposable pestle & microtube, 1.5 mL (DNase, RNase and pyrogen-free)
VWR
47747-366
These are used for tissue collection in TRIzol for expression analysis.
Dumont #5 forceps, Tips: 0.05 x 0.01mm, Inox
World Precision Instruments
500341
Fine tip dimension is desirable but is not inflexible, as one may need to sharpen the tip from time to time.
Dumont #5SF forceps, Tips: 0.025 x 0.005mm, Inox
Fine Science Tools
11252-00
Fine tip dimension is desirable but is not inflexible, as one may need to sharpen the tip from time to time.
Falcon polystyrene culture plates, 60 X 15 mm
BD Biosciences
351007
These plates are used as dissection plates.
Olympus SZX16 Stereomicroscope
Olympus
SZX16
Any stereomicroscope would work. We used Leica stereomicroscope in previous studies1-3 without any issues. We also use the 1X objective exclusively for the dissection even though we have a 2X objective installed.
Sharpening stone
Fine Science Tools
29008-01
Use this to sharpen the tip of the forceps if necessary
Thermo plate
Tokai Hit
MATS-U55SZX2B
This is used to maintain the temperature of the tissue throughout dissection and minimize the influence of temperature fluctuation on gene expression. We also put the whole microscope in an environmentally controlled room at 28°C during dissection in previous studies1-3 with good success.
Trizol, 100 mL
Invitrogen
15596-026
tungsten wire, 0.015 inch diameter
World Precision Instruments
TGW1510
Wooden Applicator
Puritan
807
This is used for holding the chemically-etched tungsten needle.