Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo

Published: April 30, 2023

Abstract

Source: Moreno, J. A., et al. Fluorescent orthotopic mouse model of pancreatic cancerJ. Vis. Exp.(2016)

This video demonstrates the protocol for implanting green fluorescent protein-expressing pancreatic cancer cells orthotopically into the pancreas of the mouse. The orthotopic mouse model helps in investigating the efficacy of novel therapeutics on primary and metastatic tumors.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the jove veterinary review board. 1. Surgical Implantation NOTE: Ensure that all surgical materials and instruments are sterile. Practice aseptic techniques at all times. Place a heating pad on the table and cover with a sterile drape. Set up the anesthesia machine and ensure all the supplies are within arm'…

Offenlegungen

The authors have nothing to disclose.

Materials

Alcare or Foamed Antiseptic Handrub Steris 639680
TB Syringe 27 G 1/2 Becton Dickinson 305620
Isoflurane Blutler Schein 50562
Ketoprofen Fort Dodge Animal Health
Surgical Scissors, 5.5" straight mayo Henry Schein 22-1600
PANC-1 GFP cell line Anticancer, Inc
Small Animal Imaging System: iBox Scientia UVP, LLC. Upland, CA. Small Animal Imaging System to observe the fluorescent tumor in live animals
SutureVet PGA 6-0 PGA Henry Schein 39010
PANC-1 GFP cell line Anticancer, Inc
DPBS (Dubelcco's PhosphateBuffered saline) Thermo Ficher Sci 21300025

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Diesen Artikel zitieren
Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo. J. Vis. Exp. (Pending Publication), e20318, doi: (2023).

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