In this video, we describe the procedure for kidney extraction from the retroperitoneal space of the mouse. The extracted kidney can further be microdissected to obtain an intact kidney without a renal capsule and the surrounding fat layer.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Kidney Dissection Anaesthetize mice by inhalation of 3–4% isoflurane in a ventilated hood. Confirm the induction of deep anesthesia by loss of toe and/or tail pinch reflex and then euthanize the mice by cervical dislocation. Apply 70% ethanol to the chest to dampen the fur. Using…
Representative Results
Figure 1: Basic kidney anatomy and location of PKJ pacemaker region. (A) Diagram of the intact kidney showing the orientation of the RP and ureter. The renal artery and renal vein are displayed in red and blue, respectively. (B) The intact kidney can be cut along a sagittal plane to expose the inner aspect of the kidney, including the medulla, papilla (dista…
Offenlegungen
The authors have nothing to disclose.
Materials
60 mm x 15 mm Petri dish
Sigma Aldrich
P5481
Kidney sharp dissection dish
Absorbent paper
Fisher Scientific
06-666A
To dry the kidney before applying
glue
B6;129S-Gt(ROSA)26Sor/J
The Jackson Laboratory
13148
GCaMP3 Mice
B6;129S-Gt(ROSA)26Sor/J
The Jackson Laboratory
24105
GCaMP6f Mice
B6.FVB-Tg(Myh11-cre/ERT2)1Soff/J
The Jackson Laboratory
19079
smMHC-CRE Mice
C57BL/6-Tg(Pdgfra-cre)1Clc/J
The Jackson Laboratory
13148
PDGFRa-CRE Mice
Ethanol
Phamco-Aaper
SDA 2B-6
For dissection
Extra-fine Bonn Scissors
Fine Science Tools
14083-08
Used for internal dissecting scissors
Fine scissors
Fine Science Tools
14060-09
Used for external dissecting scissors
Fine-tip forceps
Fine Science Tools
11254-20
Used for fine dissection of kidney
Isoflurane
Baxter
NDC 1001936060
For anesthesia
Silicon elastomer
Fisher Scientific
NC9285739
Sylgard 184
Student Adson Forceps
Fine Science Tools
91106-12
For gently holding and moving the
kidney
Student Dumont Forceps
Fine Science Tools
91150-20
Used for internal dissecting forceps
Vannas spring scissors
Fine Science Tools
15000-03
For sharp dissection and cleanup of
isolated kidney
Extracting Intact Kidney from Mouse: A Technique to Obtain Kidney Without Renal Capsule from Murine Models. J. Vis. Exp. (Pending Publication), e20549, doi: (2023).