In this video, we describe a catheter-based procedure to empty and rinse the urinary bladder of a mouse model. The prepped mouse can then be used for further experimental procedures.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Anesthetizing Animal Use a tabletop anesthesia system to anesthetize the animal with isoflurane. Turn on the O2 tank and adjust the oxygen flowmeter to 1 L/min. Place an adult female C57BL/6J mouse in the induction chamber. Turn on and adjust the isoflurane vaporizer to 3% for induction. …
Offenlegungen
The authors have nothing to disclose.
Materials
BD 1mL TB Syringe
Fisher
148232E
Exel International Disposable Safelet I.V. Catheters
Fisher Scientific
14-841-21
24G, 2.11 cm length, 0.045 cm outer diameter
K&H Heated Resting Mat for Small Animals, 9 By 12 Inches
Urine Depletion and Bladder Rinsing in Mouse Model: A Catheter-based Procedure to Empty and Wash Murine Urinary Bladder. J. Vis. Exp. (Pending Publication), e20563, doi: (2023).