This video demonstrates implantation of tumor cells in the mouse brain by performing intracranial injection.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Injecting Cancer Cells into the Brain Place an anesthetized mouse with an exposed calvarial window on the stereotatic frame. Attach an automatic injector unit to the stereotactic apparatus (Figure 1). Pull up >2 µL of cells thoroughly resuspended in 1x DPBS in a clean Hamilt…
Representative Results
Figure 1: Stereotactic and anesthesia systems. Stereotactic stand with automated syringe pump and mouse elevating box. The box is an inverted pipette tip box containing hand warmers used to elevate the mouse to the appropriate height and maintain body temperature. Inset shows the orientation of the Hamilton syringe in the automated injection apparatus.
Offenlegungen
The authors have nothing to disclose.
Materials
Bone Wax
Surgical Specialities
903
Sterile and malleable beeswax and isopropyl palmitate
Cotton tip applicators
Puritan
25-806 10WC
Sterile long stemmed cotton tip applicators
Suture
Covidien
SP5686G
45 cm swedged 5-0 monofilament polypropylene suture
Skin Glue
Vetbond
1469SB
Skin safe wounds adhesive
Stereotaxic Unit
Mouse Gas Anesthesia Head Holder
Kopf
Model 923-B
Mouth bar with teeth hole and nosecone
Non-Rupture Ear Bars
Kopf
Model 922
Ear bars suitable for mouse applications
Stereotaxic Instrument
Kopf
Model 940
Base plate, frame and linear scale assembly with digital readout monitor
Injector
Injector Needle and syringe
Hamilton
80366
26 gauge needle, 51 mm needle length and 10 μL volume syringe
Legato 130A automated Syringe Pump
KD Scientific
P/N: 788130
Programmable touch screen base with automated injector