Continuous Drug Infusion System in Mouse Model: A Surgical Procedure to Implant Micro-osmotic Pump Infusion System in the Mouse Brain for Continuous Drug Delivery

Abstract

Source: Ajoy, R., Chou, S. Y. Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain. J. Vis. Exp. (2018)

In this video, we demonstrate the assembly of a micro-osmotic pump brain infusion system followed by its surgical implantation in the mouse skull. This system enables continuous delivery of potential drugs and facilitates the study of their effects on the brain and brain tissue.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Micro-Osmotic Pump Infusion Systems NOTE: Prepare the pump, artificial cerebrospinal fluid (aCSF) buffer, and drug (Met-CCL5/RANTES protein solution (10 ng/mL in aCSF)) under sterile conditions using buffers filtered with 0.2 µm filters and conduct all the procedures un…

Representative Results

Figure 1. Osmotic pump preparation and implantation surgical procedure in mouse. (A) The brain infusion kit and pump preparation perfused with drug solution. Red arrows indicate the catheter tubes filled with liquid. (B) Fix and mount the mouse head onto the stereotactic apparatus. (C) Separate the outermost layer of the skin from the subcutaneous skin for the im…

Materials

Vetbond Tissue Adhesive	3M	#1049SB	The glue used to seal the lesion site on the mouse head
LOCTITE 454 instant adhesive	Durect Corporation	#8670	The glue used to fix the needle on the mouse skull
Alzet Micro- Osmotic Pump	Durect Corporation	#9922	0.11 μl per hour, 28 days
Brain infusion system	Durect Corporation	#8851	1-3 mm, used to perfuse the drug in to the mice brain
MIO NE116 CONTROL UNIT (nail drill)	Mio System	#E235-015	To drill a hole in the skull of the mouse
aCSF formula	119 mM NaCl     26.2 mM NaHCO3 2.5 mM KCl               1 mM NaH2PO4   1.3 mM MgCl2       10 mM glucose		Filter sterilize with a 0.22 μm filter apparatus, and store at 4°C. aCSF is stable for 3-4 weeks