Generating Urinary Tract Infection Model: A Technique to Administer Uropathogenic Bacteria in Mouse to Develop Urinary Tract Infection Model

Abstract

Source: Luyts, N. et al., Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging. J. Vis. Exp. (2021).

In this video, we describe the technique for the intravesical administration of uropathogenic bacteria to generate a urinary tract infection model in mice. This model can be used to understand the spread of uropathogenic bacterial infection within the urinary system and the mechanisms that determine UTI susceptibility.

Protocol

1. Inoculation of the animals Preparation of the animals Choose the desired mouse strain(s) depending on the research question and availability of knock-out lines, experimental details, and differences in UTI susceptibility. Keep in mind that transurethral catheterization is easier in female mice. Do not use animals younger than 8 weeks, as they are immunologically immature. Here, 12-week-old female C57Bl/6J mice were used. Order animals well in advance and let them acclimate, ideally f…

Materials

Aesculap ISIS	Aesculap	GT421	hair trimmer, with GT608 cap
Anesthesia vaporizer	Harvard apparatus limited	N/A	https://www.harvardapparatus.com/ harvard-apparatus-anestheticvaporizers.html
BD Insyte Autoguard 24 GA	BD	382912	Yellow angiocatheter, use sterile plastic tip for instillation
C57Bl/6J mice	Janvier	N/A
Dulbecco's Phosphate Buffered Saline, Gibco	ThermoFisher Scientific	REF 14040-083
Ethanol 70% denaturated 5L	VWR international	85825360
Hamilton GASTIGHT syringe, PTFE luer lock, 100 µL	Sigma-Aldrich	26203	to ensure slow bacterial instillation of 50 µL
Iso-Vet 1000mg/g	Dechra Veterinary products	N/A	Isoflurane