A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria

Abstract

Source: Keil, S. D., et al. Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination. J. Vis. Exp. (2015).

This video demonstrates the riboflavin and UV light-based pathogen reduction process against high-titer bacterial contamination in human platelet products. This process could help to lower the risk of severe adverse transfusion events associated with bacterial contamination.

Protocol

1. Platelet Product Preparation Collect a standard apheresis human platelet product at an accredited blood bank. The platelet product must meet the following collection specifications: 90–360 ml volume and 0.8 –3.8 x 106 platelets/µl concentration. Ensure that the platelet product has rested for a minimum of 2 hr prior to processing for pathogen reduction. Store the products at 22 ± 2 °C in a platelet incubator without agitation for these 2 hr…

Materials

Mirasol Illuminator	Terumo BCT	N/A
Mirasol Treatment/Storage Bag	Terumo BCT	N/A
Hematology Analyzer	Beckman-Coulter	Ac•T diff
Blood Gas Analyzer	Siemens	RapidLab 1265
Sterile Docking Device	Terumo BCT	TSCD
Platelet Incubator	Helmer	PC2200
Orbital Incubator Shaker	Lab-Line	4628
Dry Incubator	Fisher	Iso-temp
Class II A/B3 Biosafety Cabinet	Thermo-forma	1286
Tubing Sealer	Sebra	Smart Sealer II
Table Top Centrifuge	IEC	Centra GP8R
10 ml Syringe	BD	309604
30 ml Syringe	BD	309650
5 ml Dilution Tube	VWR	211-0058
50 ml Conical Tube	Corning	430290
Micropipette	Gilson	P-200
Micropipette	Rainin	R-200
25 ml Disposable Serological Pipette	Costar	4489