This video demonstrates a method for generating an mRNA vaccine candidate using polymeric nanoparticles. The cationic polymer interacts with negatively charged mRNA, forming polyplexes. The formation of polyplexes stabilizes the mRNA, making it a potential candidate for future mRNA vaccines in immunotherapeutic applications.
Protocol
1. Synthesis of poly(beta amino esters) (pBAE) polymer with end oligopeptides (OM-pBAE) Polymerization of C6-pBAE Add 5-amino-1-pentanol (38 mmol; MW = 103.16 Da) 1-hexylamine (38 mmol; MW = 101.19 Da) into a round-bottom glass flask (100 mL). Then, add 1,4-butanediol diacrylate (82 mmol; MW = 198.22 Da). Pre-heat the silicone oil bath at 90 °C, place the round-bottom flask into the oil bath and stir the mixture with the aid of a magnetic stir bar overnight (~…
Offenlegungen
The authors have nothing to disclose.
Materials
1,4-butanediol diacrylate
Sigma Aldrich
123048
1-hexylamine
Sigma Aldrich
219703
5-amino-1-pentanol
Sigma Aldrich
411744
Acetone
Panreac
141007
Chlor hydroxhyde
Panreac
181023
Chloroform-d
Sigma Aldrich
151823
Cys-His-His-His peptide
Ontores
Custom
Cys-Lys-Lys-Lys peptide
Ontores
Custom
D2O
Sigma Aldrich
151882
DEPC reagent for Rnase free water
Sigma Aldrich
D5758
This reagent is important to treat MilliQ water to remove any RNases of the buffers