Materials
| Name | Company | Catalog Number | Comments |
| Restriction enzymes | New England Biolabs | Various | |
| Jet Prime transfection kit | Polyplus | 114-01 | |
| DMEM | Gibco (Life Technologies) | 10567014 | |
| E. coli BJ5183 bacteria | Agilent | 200154 | |
| PD-10 desalting column | GE Healthcare | 17-0851-01 | |
| EmeraldAmp polymerase | Takara | RR320A | |
| Nucleospin plasmid kit | Macherey-Nagel | 740558.250 | |
| NucleoSpin Gel and PCR Clean-up | Macherey-Nagel | 740609.50 | |
| 14 ml tubes, Ultra Clear | Beckman | 344060 |
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