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Kapitel
- 00:05Titel
- 00:54Introduction
- 01:23Biotinylation of DNA and Labeling of the Cy5-Cationic Polymer
- 05:51Preparing the QD-labeled DNA and Cy5-Polymer
- 07:15Fabricating the SU-8 Masters Using Standard Photolithography
- 10:07Replica Molding of PDMS from the Masters and Bonding to the Cover Glass
- 12:16Monitoring the Formation of DNA Nanocomplexes in the Microfluidic Device
- 13:30Results of QD-FRET-Indicated DNA Nanocomplex Self-Assembly
- 14:04Conclusion
Biz gelecekteki nükleik asit temelli tedaviler için daha iyi kontrol ve sentez üniforma ve özelleştirilebilir polyplexes sağlaması bekleniyor polielektrolit polyplexes oluşumunu araştırmak için yeni ve güçlü bir entegrasyon sahipliği yapacaktır (QD-FRET) ve Mikroakiskan sunuyoruz.
Tags
QD-FRETMicrofluidicsDNA Nanocomplex Self-assemblyGenomicsTherapeuticsIntracellular Delivery SystemGene CarrierElectrostatic Self-assemblyCellular UptakeDegradation ProtectionRational DesignPremature DissociationStable BindingSelf-assembly KineticsPhysical PropertiesTransfection EfficienciesBioactivitiesNanophotonics
