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Journal
/
Biologie
/
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
/
Crypt Isolation from Murine Small Intestine
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
Crypt Isolation from Murine Small Intestine
Crypt Isolation from Murine Small Intestine
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
DOI:
10.3791/200007-v
•
05:06 min
•
June 02, 2023
•
Yuta Takase
,
Kazuto Fujishima
3
,
Toshio Takahashi
1
Suntory Foundation for Life Sciences
,
Bioorganic Research Institute
,
2
Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS)
,
Kyoto University
,
3
Faculty of Medicine
,
Osaka Medical and Pharmaceutical University
Tags
Crypt Isolation
Murine Small Intestine
PBS Antibiotics
EDTA
Extracellular Matrix
ECM
DMEM
Sorbitol
Cell Strainer
Cell Culture
Centrifugation
Microscopy
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The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
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