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Immunologie und Infektion
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Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses
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SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay

SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay

Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses

DOI:
10.3791/200835-v

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04:50 min

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November 21, 2023

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, , , , , , , , , , , ,
1Department of Neuroscience, Biomedicine and Movement,University of Verona, 2Laboratory of Molecular Neurovirology, Faculty of Health Science,University of Brasília, 3Viral Pseudotype Unit, Medway School of Pharmacy,Universities of Kent and Greenwich at Medway

Tags

SARS-CoV-2Pseudotype VirusSerum Neutralization AssayHEK 293T CellsTransfectionPolyethylenimineCell CultureVirus ProductionViral SupernatantSerial DilutionHEK 293T ACE2 CellsLuciferase AssaySerum SamplesHeat Inactivation

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