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Immunology and Infection

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JoVE Journal Immunology and Infection
Determining the Phagocytic Activity of Clinical Antibody Samples
 

Determining the Phagocytic Activity of Clinical Antibody Samples

Article DOI: 10.3791/3588-v • 09:06 min • November 30th, 2011
November 30th, 2011 •
1, 1, 1, 1, 1, 2
1Massachusetts General Hospital, Ragon Institute of MGH, MIT, and Harvard, 2Thayer School of Engineering, Dartmouth College

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Summary
Automatic Translation

We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.

Tags

Phagocytic Activity Clinical Antibody Samples Fc Receptors Clearance Pathology Of Disease Variable Domains Innate Immune Cells Recombinant Monoclonal Antibody Therapy Natural Infection Vaccination IgG Subclass Glycosylation Functional Differences Antigen-specific Antibodies Disease State Susceptibility To Infection Progression Clinical Outcome Effector Function Enhance Infection Fc Receptor-driven Phagocytosis Immune Complexes Th1/Th2 Polarization Assay
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