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Neuroscience

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JoVE Journal Neuroscience
Wholemount Immunohistochemistry for Revealing Complex Brain Topography
 

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

Article DOI: 10.3791/4042-v • 09:24 min • April 5th, 2012
April 5th, 2012 •
1, 1, 2, 1
1Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University, 2Department of Cell Biology and Anatomy and the Hotchkiss Brain Institute, Faculty of Medicine, University of Calgary

Kapitel

Summary
Automatic Translation

Neural circuits are topographically organized into functional compartments with specific molecular profiles. Here, we provide the practical and technical steps for revealing global brain topography using a versatile wholemount immunohistochemical staining approach. We demonstrate the utility of the method using the well-understood cytoarchitecture and circuitry of cerebellum.

Tags

Wholemount Immunohistochemistry Brain Topography Cerebellum Cellular Architecture Gene Expression Domains Protein Expression Domains Molecular Compartmentalization Afferent Fibers Patterning Defects Mouse Cerebellum Wholemount Staining Approach Protein Expression Patterns ZebrinII/aldolaseC Expression Three-dimensional Conformation Afferent Projections Comparative Studies Molecular Topography Rat Cerebellum
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