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Immunology and Infection

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JoVE Journal Immunology and Infection
In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
 

In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers

Article DOI: 10.3791/51679-v • 13:10 min • August 1st, 2014
August 1st, 2014 •
1, 1, 1, 2, 3, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, 2Center for Biotechnology, University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln

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Summary
Automatic Translation

The protocol to detect self-reactive CD4 T cells in brain and heart by direct staining with major histocompatibility complex class II dextramers has been described in this report. For comprehensive analysis, a reliable method to enumerate the frequencies of antigen-specific CD4+ T cells in situ is also devised.

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Major Histocompatibility Complex Class II Dextramers Autoreactive CD4 T Cells Brain Heart Detection In Situ Myelin Proteolipid Protein Experimental Autoimmune Encephalomyelitis SJL Mice Cardiac Myosin Heavy Chain-alpha Experimental Autoimmune Myocarditis A/J Mice Dextramer Reagents Laser Scanning Confocal Microscope EAE IAs/PLP 139-151 Dextramers Anti-CD4 IAs/Theiler's Murine Encephalomyelitis Virus TMEV 70-86 Dextramers EAM IAk/Myhc 334-352 Dextramers IAk/bovine Ribonuclease (RNase) 43-56 Dextramers LSCM Analysis Brain Sections Heart Sections PLP 139-151 Dextramers Staining Myhc 334-352-dextramer+ Cells
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