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Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture
 

Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture

Article DOI: 10.3791/51969-v • 11:49 min • August 3rd, 2014
August 3rd, 2014 •
1,2, 3, 3, 3
1Graduate School of Medicine, Jichi Medical University, 2Division of Biology, Center for Molecular Medicine, Jichi Medical University, 3Department of Developmental Neuroscience, United Center for Advanced Research and Translational Medicine (ART), Tohoku University School of Medicine

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Summary
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Mammalian whole embryo culture (WEC) is widely used in teratology and developmental biology. Immediately centrifuged rat serum is commonly provided as a medium for both mouse and rat WEC. In this video, we demonstrate our standard protocol for the preparation of high-quality rat serum suitable for mammalian WEC.

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Rat Serum Mammalian Whole Embryo Culture Pharmacological Toxicity Developing Mouse Embryos Rat Embryos Mechanisms Of Developmental Processes Centrifuged Rat Serum Cultured Mouse Embryos Midgestation Embryos Rat Serum Preparation Blood Collection Plasma Fraction Fibrin Clot Sterile Forceps Extraction Of Serum Centrifugation
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