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冲红灯光感受器原子力显微镜使用PeakForce定量纳米机械性能映射

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Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

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冲红灯光感受器原子力显微镜使用PeakForce定量纳米机械性能映射

DOI:

10.3791/52164-v

•

14:13 min

•

October 24, 2014

•

Marie E. Kroeger, Blaire A. Sorenson, J. Santoro Thomas, Emina A. Stojković, Stefan Tsonchev, Kenneth T. Nicholson

¹Department of Biology,Northeastern Illinois University, ²Department of Chemistry,Northeastern Illinois University

Kapitel

00:05Titel
02:27Computer and Microscope Setup
03:45Fluid Cell Assembly and Imaging of Mica Surface
08:25Protein Deposition onto the Mica
10:08Imaging Proteins on Mica
12:20Results: AFM Images of Photoreceptor P3
13:14Conclusion

Summary

Automatische Übersetzung

A method for investigating the structure of a protein photoreceptor using atomic force microscopy (AFM) is described in this paper. PeakForce Quantitative Nanomechanical Property Mapping (PF-QNM) reveals intact protein dimers on a mica surface.

Tags

Atomic Force Microscopy AFM PeakForce Quantitative Nanomechanical Property Mapping PF-QNM Red-Light Photoreceptor RpBphP3 P3 Rhodopseudomonas Palustris Biological Macromolecules Soft Materials

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