Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / An Ex Vivo Laser-induced Spinal Cord Injury Model …
JoVE Journal
Neuroscience

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Neuroscience
An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time
 

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Article DOI: 10.3791/52173-v • 11:18 min • November 25th, 2014
November 25th, 2014 •
1, 1, 2, 1
1KY Spinal Cord Injury Research Center, Department of Neurological Surgery, University of Louisville, 2Hotchkiss Brain Institute, Department of Clinical Neurosciences, University of Calgary

Kapitel

Summary
Automatic Translation

We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.

Tags

Ex Vivo Laser-induced Spinal Cord Injury Mechanisms Of Axonal Degeneration Real-time Assessment CNS Axons Regeneration Failure Secondary Axonal Degeneration Growth Cone Formation Myelinated Axonal Projections Spinal Cord Model Transgenic Mice Yellow Fluorescent Protein Nile Red Dye Two-photon Excitation Time-lapse Microscopy Acute Axonal Injury Axonal Retraction Myelin Degeneration Contusion-based Injuries In Vivo Spinal Cord Imaging Primary And Secondary Axonal Injury Responses High Resolution Microscopy Contusion/compression-induced Axonal Pathologies
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter