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Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
 

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting

Article DOI: 10.3791/52227-v • 11:31 min • November 30th, 2015
November 30th, 2015 •
1,2, 3, 1,4, 5, 6, 7, 6, 1,2
1Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 2Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, 3Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, 4Department of Psychology, University of Illinois at Urbana-Champaign, 5Department of Epidemiology, Mailman School of Public Health, Columbia University, 6Department of Epidemiology, University of Michigan School of Public Health, 7Center for Molecular Medicine and Genetics, Wayne State University School of Medicine

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Summary
Automatic Translation

We outline a methodology for the processing of whole blood to obtain a variety of components for further analysis. We have optimized a streamlined protocol that enables rapid, high-throughput simultaneous processing of whole blood samples in a non-clinical setting.

Tags

Rapid Fractionation Isolation Whole Blood Components Samples Community-based Setting Collection Processing Non-clinical Setting Evaluate Community-dwelling Individuals Preexisting Conditions Degradation Cellular Components Peripheral Blood Mononuclear Cell (PBMC) DNA Isolation RNA Isolation Serum Isolation Blood Draw Homes Of Consenting Participants Metropolitan Area Processing Initiated Within 2 Hr Of Collection Blood Specimens High Quality Material DNA Methylation Analysis Genotyping Analysis Gene Expression Analysis Flow Cytometry Analysis Standard Methods Efficient Processing Population-based Studies
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