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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry
 

In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry

Article DOI: 10.3791/52736-v • 07:16 min • April 5th, 2015
April 5th, 2015 •
1, 1, 1,2, 1
1Australian School of Advanced Medicine, Macquarie University, 2John Curtin School of Medical Research, Australian National University

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Summary
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The malaria parasite invades and replicates within red blood cells. The accurate assessment of merozoite invasion and parasitemia is therefore crucial in assessing the course of malaria infection. Here we describe a flow cytometry based protocol for the measurement of these parameters in a mouse model of malaria.

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In Vivo Assessment Rodent Plasmodium Parasitemia Merozoite Invasion Flow Cytometry Blood Stage Infection Malaria Parasites Red Blood Cells (RBCs) Parasitemia DNA Dye Hoechst Mitochondrial Membrane Potential Dye JC-1 Plasmodium Chabaudi Adami DS Infected Mice Fluorescently Conjugated Antibodies Leukocytes RBC Progenitors Howell-Jolly Bodies (HJ-RBCs) Comparative Assessment Merozoite Invasion Assay Transfused RBCs Flow Cytometry Analysis In Vivo Model Of Malaria Infection
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