Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Optimization of the Wound Scratch Assay to Detect …
JoVE Journal
Developmental Biology

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Developmental Biology
Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
 

Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

Article DOI: 10.3791/53414-v • 08:40 min • December 3rd, 2015
December 3rd, 2015 •
*1, *1, 1, 1
1Department of Biology, College of the Holy Cross
* These authors contributed equally

Kapitel

Summary
Automatic Translation

The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.

Tags

Wound Scratch Assay Cell Migration Mesenchymal Stromal Cells Soluble Cigarette Smoke Extract Optimization Quantifying Migratory Capacity Tissue Repair Scratch Assays Trans-well Migration Assays Boyden Chambers Micropillar Arrays Cell Exclusion Zone Assays Reproducibility Customizability Quantification Cost-effectiveness Cell Microenvironment Senescent Cells Cell Proliferation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter