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A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side
 

A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side

Article DOI: 10.3791/54061-v • 09:58 min • May 6th, 2016
May 6th, 2016 •
1, 1, 1, 2, 1, 1, 1
1Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, 2Department of NDU Life Sciences, Nippon Dental University

Kapitel

Summary
Automatic Translation

The epithelial cells of the choroid plexus (CP) form the blood-cerebrospinal fluid barrier (BCSFB). An in vitro model of the BCSFB employs human choroid plexus papilloma (HIBCPP) cells. This article describes culturing and basolateral infection of HIBCPP cells using a cell culture filter insert system.

Tags

Choroid Plexus Epithelial Cell-based Model Human Blood-cerebrospinal Fluid Barrier Bacterial Infection Basal-lateral Side Infectious Diseases Choroid Plexus Epithelium Pathogens Transmigration Immune Cells Tumor Cells HIBCCP Cells Growth Area Cell Culture Filter Inserts Pre-warmed Medium Subculture Medium Supplement
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