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JoVE Journal Medicine
Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs
 

Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs

Article DOI: 10.3791/54077-v • 10:47 min • July 2nd, 2016
July 2nd, 2016 •
1,2, 1, 1, 1, 1, 1, 1, 1
1College of Veterinary Medicine, Gyeongsang National University, 2PWG Genetics Pte Ltd.

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Summary
Automatic Translation

A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.

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Isolation Cellular Phenotyping Mesenchymal Stem Cells Synovial Fluid Bone Marrow Minipigs Non-invasive Collection Regeneration Immunomodulation Studies Otologist MSC Therapy Synovial Fluid MSC Characterization Joint Diseases Athletic Patients Regenerative Medicine Autoimmune Disease Research Immunosuppression Hyeon-Jeong Lee Won-Jae Lee Iliac Crest Access Depilation Povidone-iodine Scrub
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