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Developmental Biology

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JoVE Journal Developmental Biology
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
 

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Article DOI: 10.3791/54253-v • 11:06 min • March 21st, 2017
March 21st, 2017 •
*1, *2, 3, 3
1Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, 2Indiana Center for Biological Microscopy, Department of Medicine, Indiana University School of Medicine, 3Department of Pediatrics, Indiana University School of Medicine
* These authors contributed equally

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Summary
Automatic Translation

Intravital fluorescence microscopy (IVFM) of the calvarium is applied in combination with genetic animal models to study the homing and engraftment of hematopoietic cells into bone marrow (BM) niches.

Tags

Intravital Fluorescent Microscopy IVFM Genetic Models Engraftment Dynamics Hematopoietic Cells Bone Marrow Niches Visualization Measurement Transplanted Blood Cells Cellular Processes Homing Expansion Localization Donor Hematopoietic Cells Transplantation Engineered Animal Models Molecular Pathways Histology Imaging Techniques Cell-to-cell Interactions Live Animal Real Time Bone Marrow Microenvironment Myeloproliferative Disease Notch Signaling Loss Breathing Artifact Head Positioning And Stabilization Image Saturation
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