Journal
/
/
Cytoplasmique microinjection dans zygotes Bétail assistée par laser
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
DOI:

08:59 min

October 05, 2016

, ,

Kapitel

  • 00:05Titel
  • 00:42Formation of the Injection Micropipette
  • 02:19Formation of the Holding Micropipette
  • 03:29Micromanipulator Setup
  • 04:03Preparation of the Injection Dish
  • 04:43Microinjection
  • 07:30Results: High Success Rate with Laser-assisted Cytoplasmic Microinjection
  • 08:19Conclusion

Summary

Automatische Übersetzung

This protocol shows how to perform cytoplasmic microinjection in farm animal zygotes. This technique can be used to deliver any solution into the one-cell embryo such as genome editing tools to generate knockout animals.

Verwandte Videos

Read Article