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Journal / Neuroscience / Patch Clamp Recording of Starburst Amacrine Cells …
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Neuroscience

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JoVE Journal Neuroscience
Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina
 

Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina

Article DOI: 10.3791/54608-v • 08:44 min • October 13th, 2016
October 13th, 2016 •
1, 1,2, 1, 1,2,3
1Department of Ophthalmology, Baylor College of Medicine, 2Department of Neuroscience, Baylor College of Medicine, 3Department of Biochemistry and Molecular Biology, Baylor College of Medicine

Kapitel

Summary
Automatic Translation

This protocol demonstrates how to perform whole-cell patch clamp recording on retinal neurons from a flat-mount preparation.

Tags

Patch Clamp Recording Starburst Amacrine Cells Flat-mount Preparation Deafferentated Mouse Retina Retinal Neurobiology Synaptic Connections Vertical Connections Lateral Connections Delicate Tissue Carbogenated Mammalian Ringer Solution Limbus Micro Scissors Cornea And Lens Removal Retinal Whole-mount Pigmented Epithelium Orthogonal Cuts Nitrocellulose Membrane Optic Nerve Head
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