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JoVE Journal Medicine
Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation
 

Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation

Article DOI: 10.3791/55495-v • 08:05 min • July 3rd, 2017
July 3rd, 2017 •
*1,2,3, *2, 2, 2, 1, 2,4,5
1National Healthcare Group Eye Institute, Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), Singapore National Eye Center, 3School of Material Science and Engineering, Nanyang Technological University, 4Department of Ophthalmology, Yong Loo Lin School of Medicine, National University Health Systems, National University of Singapore, 5Ophthalmology Academic Clinical Research Program, DUKE-NUS Graduate Medical School
* These authors contributed equally

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Summary
Automatic Translation

Live-cell imaging of the labeled blood cells in ocular circulation can provide information about inflammation and ischemia in diabetic retinopathy and age-related macular degeneration. A protocol to label blood cells and image the labeled cells in the retinal circulation is described.

Tags

Fluorescent Dye Labeling Erythrocytes Leukocytes Flow Dynamics Mouse Retinal Circulation Scanning Laser Ophthalmoscope Retinal Diseases Disease Pathogenesis Disease Phenotypes Easy To Perform Blood Cells Layer Polysucrose Sodium Diatrizoatem Solution Centrifuge Plasma Supernatant Buffy Coat Layer Leukocytes Erythrocytes PBS Microcentrifuge Tubes Aliquots 40%1XPBS Incubate ICG
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