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Neuroscience

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JoVE Journal Neuroscience
Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins
 

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Article DOI: 10.3791/55497-v • 08:39 min • May 22nd, 2017
May 22nd, 2017 •
*1, *1, 1, 1
1Experimental Biophysics, Institute of Biology, Humboldt-Universität zu Berlin
* These authors contributed equally

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Summary
Automatic Translation

This article describes how the ion selectivity of channelrhodopsin is determined with electrophysiological whole-cell patch-clamp recordings using HEK293 cells. Here, the experimental procedure for investigating chloride selectivity of an anion-selective channelrhodopsin is demonstrated. However, the procedure is transferable to other channelrhodopsins of distinct selectivity.

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Whole-cell Patch-clamp Recordings Electrophysiological Determination Ion Selectivity Channelrhodopsins Chloride Selectivity Light-gated Ion Channels Biophysics Photocurrent Amplitudes Kinetics Direct Readout Biophysical Channel Activity Experimental Procedure HEK Cells Seeding Transection Intracellular Buffered Solution Extracellular Buffered Solution Osmolarity Glucose Lysine-coated Glass Cover Slips Petri Dish Transfection
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