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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Genetically Modified Mice through the Microinjection of Oocytes
 

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Article DOI: 10.3791/55765-v • 10:19 min • June 15th, 2017
June 15th, 2017 •
1, 1
1Transgenic Animal Unit, Mark Wainwright Analytical Centre, University of New South Wales

Kapitel

Summary
Automatic Translation

The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.

Tags

Genetically Modified Mice Microinjection Oocytes Mouse Genesis Gene Targeting Single Guide RNA PCR Purification Kit PCR Product Binding Buffer Wash Buffer Centrifuge
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