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Journal / Genetics / An Ecdysone Receptor-based Singular Gene Switch…
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Genetics

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JoVE Journal Genetics
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
 

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Article DOI: 10.3791/57494-v • 06:21 min • May 7th, 2018
May 7th, 2018 •
*1, *2, 3, 2, 1
1Department of Biological Sciences, College of Bioscience and Biotechnology, Chungnam National University, 2Department of Pharmacology, College of Medicine, Chungnam National University, 3Department of Nursing, Gwangju Women's University
* These authors contributed equally

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Summary
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Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.

Tags

Ecdysone Receptor Gene Switch Transgene Expression Chemical Inducer Tebufenozide Cell Culture HEK 293 Cells Transfection Reagent DNA Incubation Teb
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